The MAGUS Lum V500 inverted fluorescence microscope is designed for observing samples in laboratory glassware with a height of up to 55 mm and a bottom thickness of up to 1.2 mm. By tilting the illuminator support, it becomes possible to use larger laboratory glassware, up to 165 mm. Samples can be observed in reflected light (fluorescence microscopy technique) and in transmitted light (with bright field and phase contrast microscopy). The main fields of application of this microscope are: medicine, pharmacology, biochemistry, epidemiology, etc.
Digital camera
The MAGUS CLM90 digital camera is designed for the dark field and fluorescence microscopy technique.
The camera is equipped with a 7.1 MP sensor and produces realistic images at a resolution of 3200x2200 px. It is recommended to use the camera with 4x, 10x, 20x and 40x lenses. When using low magnification lenses, the camera allows the user to see sharper details.
Videos are recorded at 51.3 fps or 133.8 fps depending on the resolution. Videos flow smoothly with smooth, subtle transitions between frames. The movement of the sample is shown in real time, without delays. The camera facilitates the observation of moving objects and is suitable for academic demonstrations.
The camera is equipped with a USB 3.0 interface. The data transfer speed is 10 times faster than USB 2.0 cameras. The high-speed camera is recommended for professional laboratories, research or university education.
Optics
This microscope is equipped with a trinocular head with a vertical optical tube . There is a side camera port on the microscope frame. This design choice allows you to simultaneously install a digital camera and a monitor on the microscope. The beam splitter of the trinocular head has a ratio of 80/20, while that of the microscope frame is 100/0 and 0/100. The eyepiece tubes are rotatable through 180°, which allows the eye relief to be adjusted to suit the user.
The nosepiece is placed on a stand underneath the stage. It is equipped with six free slots, for housing as many lenses. The package includes three objectives for phase contrast and three objectives for fluorescence and brightfield observations. These are planachromatic objectives with a long working distance, designed for laboratory glassware with a 1.2 mm thick bottom.
Illumination
The source of Reflected light is a 100W mercury vapor lamp. It is placed in a lamp holder for better heat dissipation and less risk of overheating during extended use. This lamp emits light in a wide range of wavelengths with various peak intensities, making it perfect for working with a variety of fluorophores. Fluorescence filters: ultraviolet (UV), violet (V), blue (B), and green (G). The mercury vapor lamp illuminates the sample intensely, providing good visibility and can be easily replaced when necessary.
The transmitted light is provided by a 30 W halogen lamp. The illumination provided is intense and with a warm color temperature. As a result, observation through each of the lenses is always efficient and comfortable for the eyes. The microscope has a phase contrast condenser with four slots: one is used for brightfield observations and the other three are designed for 10x, 20x and 40x phase contrast objectives. Phase contrast rings can be centered. To switch from bright field observation to phase contrast techniques, and vice versa, simply rotate the disk. This is particularly practical for professional researchers, as it saves time.
Application of Köhler illumination for transmitted and reflected light observations. Using this method allows you to obtain clear and sharp images, in high resolution, without artifacts or darkening of the edges.
Focusing stage and mechanism
The stage is fixed and is equipped with a mechanism for moving the laboratory glassware on which the sample is positioned. Laboratory glassware can be moved along the horizontal plane, along two axes perpendicular to each other. The movement is smooth and precise and the mechanism includes four holders for accommodating glassware of various sizes. The sharpness of the microscope image is adjusted by rotating the coarse and fine focus knobs (macro and fine screw). The coarse focus is equipped with a fixing knob and the tension of the coarse screw can be adjusted. The focus knobs are coaxial and are located on both sides of the frame.
Accessories
This product line includes additional accessories for the microscope MAGUS Lum V500: eyepieces, digital cameras and calibration slides.
Key features of the microscope:
- Fluorescence microscopy in reflected light and microscopy in bright field and phase contrast in transmitted light
- Possibility of observing samples inside laboratory glassware with a height of up to 55 mm, or 165 mm with inclined support
- lamp 100 W mercury vapor lamp for reflected light observations, 30 W halogen lamp for transmitted light observations, configurable Köhler illumination
- Four fluorescence filters: ultraviolet (UV), violet (V), blue (B) and green (G)
- 180° rotatable trinocular head. Two options for installing a digital camera and monitor: 80/20 beam splitter (trinocular head) or 100/0 or 0/100 beam splitter (microscope frame)
- Stage with slots for Petri dishes and laboratory glassware of various diameters
- Phase contrast condenser with bright field aperture and three phase contrast apertures
Key camera features:
- For darkfield and fluorescence microscopy with 4x, 10x, 20x or 40x objectives
- 7.1 MP resolution: when performing observations with low magnification, the camera will allow the user to see sharper details
- 51.3 fps and 133.8 fps, respectively at 3200x2200 pixels and 1584x1100 pixels resolution, for observing objects with moving elements , for video recording and for moving the specimen without jerks or delays
- The Peltier cell lowers the sensor temperature during prolonged operation and eliminates thermal noise, thus allowing the use of a long speed of the shutter when taking photographs
- Global shutter for fast reading of the signal by the sensor, increased image brightness without optical effects when observing moving objects
- The CMOS sensor SONY Exmor monochrome backlit delivers images with low noise and high light sensitivity even in low light conditions. You can get sharper, brighter and more saturated color images
- USB 3.0 interface for fast and smooth data transfer
- Software for recording and editing photos and videos, display functions external, linear and angular measurements
The package contains:
- MAGUS CLM90 digital camera (digital camera, USB cable, adapter 12 V, 3 A, case, installation CD with drivers and software, user manual and warranty card)
- Base with power input, transmitted light light source and condenser, focusing mechanism, stage and nosepiece
- Fluorescence unit
- Mercury vapor lamp housing
- Trinocular head
- Infinity planachromatic objective: PLL 10x/0.25 WD 4.3 mm
- Plan achromatic infinity objective: PLL 20х/0.40 WD 8.0 mm
- Plan achromatic infinity objective: PLL 40х /0.60 WD 3.5 mm
- Plan achromatic infinity objective: PLL 10x/0.25 PHP2 WD 4.3 mm
- Plan achromatic infinity objective: PLL 20x/ 0.40 PHP2 WD 8.0 mm
- Infinity plan achromatic objective: PLL 40x/0.60 PHP2 WD 3.5 mm
- 10x/22 mm eyepiece with long eye relief (2 pcs.)
- UV screen
- 1x C-mount adapter
- Allen key
- Mercury vapor lamp power supply
- Power cord
- Reflected light illuminator power cord
- Dust cover
- User manual and warranty card
Available on request:
- 10x/22 mm eyepiece with graduated scale
- 12.5x/14 mm eyepiece (2 pcs.)
- Eyepiece 15x/15 mm (2 pcs.)
- Eyepiece 20x/12 mm (2 pcs.)
- Eyepiece 25x/9 mm (2 pcs.)
- Calibration glass
| Brand | MAGUS |
| Warranty | 5 лет |
| EAN | 5905555018218 |
| Package size (LxWxH) | 38x61x82 cm |
| Shipping weight | 28.7 kg |
| Delimiter | Inverted fluorescence microscope MAGUS Lum V500 |
| Type | biological, light/ optical |
| Head | trinocular |
| Vertical tube | Siedentopf, rotatable at 180° |
| Magnification, x | 100–400 in the basic configuration (*optional: 40–500/600/800/1000) |
| Eyepiece tube diameter, mm | 30 |
| Eyepieces | 10х/22 mm , eye relief: 10 mm (*optional: 10x/22 mm with graduated scale, 12.5x/14; 15x/15; 20x/12; 25x/9) |
| Objectives | plan achromatic objectives at infinity (∞): PLL 10x/0.25/4.3; PLL 20x/0.40/8.0; PLL 40x/0.60/3.5; phase contrast: PLL 10x/0.25/4.3 PHP2; PLL 20x/0.40/8.0 PHP2; PLL 40x/0.60/3.5 PHP2; parfocal distance: 45 mm |
| Nosepiece revolver | for 6 objectives |
| Working distance, mm | 4.3 (10х); 8.0 (20x); 3.5 (40x) |
| Interpupillary distance, mm | 48 — 75 |
| Stage, mm | 227x208 |
| Range of movement of the stage, mm | 77/134.5 |
| Table features | fixed, with Ø118 mm glass plate and mechanical slider; plate holder: 86x129.5, Ø90 mm; 34x77.5 mm, Ø68.5 mm; 57x82 mm, Ø60 mm; 29x77.5 mm, Ø35 mm |
| Capacitor | NA 0.6; working distance: 55 mm; phase contrast turret; with fixing screws |
| Diaphragm | adjustable aperture diaphragm, field diaphragm with adjustable iris |
| Focus | coaxial, coarse focus (with micrometer screw tension adjustment knob and fixing knob) and fine focus (0.002 mm micrometer screw) |
| Lighting | halogen, fluorescent |
| Brightness adjustment | yes |
| Power supply | AC mains, 85–265 V, 50/60 Hz |
| Type of light source | reflected light: 100 W mercury vapor lamp; transmitted light: 12 V halogen lamp, 30 W |
| Optical filters | yes |
| Operating temperature range, °C | 5 — 35 |
| Special features | phase contrast condenser (turret) with available housing and phase contrast ring plates for 10x, 20x, and 40x objectives; auxiliary centering microscope |
| User level | expert users, professionals |
| Level of difficulty assembly and installation | complicated |
| Fluorescent module | filters: ultraviolet (UV), violet (V), blue (B), green (G) |
| Fluorescence filter: filter type/excitation wavelength/dichroic mirror/emission wavelength | ultraviolet ( UV), 320–380 nm/425 nm/435 nm; violet (V) 380–415 nm/455 nm/475 nm; blue (B) 450–490 nm/505 nm/515 nm; green (G) 495–555 nm/585 nm/595 nm |
| Application | laboratory/medical |
| Illumination position | double |
| Research method | bright field, phase contrast microscope, fluorescence |
| Set with pencil case/case/bag | dust cover |
| === Delimiter ===< /td> | Digital camera |
| Sensor | SONY Exmor CMOS |
| Color/ monochrome | monochrome |
| Megapixel | 7.1 |
| Maximum resolution, px | 3200x2200 |
| Sensor size | 1.1" (14.4x9.9 mm) |
| Pixel size, µm | 4.5x4.5 |
| Two-phase thermoelectric module (Peltier cell) to set the temperature at 42°C below room temperature | yes |
| Sensitivity to light | 3354 mV at 1/30 s< /td> |
| Signal-to-noise ratio | 0.15 mV at 1/30 s |
| Exposure time | 0.1 ms – 1 h |
| Video recording | yes |
| 51.3@3200x2200, 133.8@1584x1100 |
| ADC digital capacity (bit) | 8/12 (selectable) |
| Image format | *.jpg, *.bmp, *.png, *.tif td> |
| Video format | recording: *.wmv, *.avi, *.h264 (Windows 8 and later), *h265 (Windows 10 and later)< /td> |
| Spectral range, nm | 380–650 (with IR filter and anti-reflection filter) |
| Type shutter speed | Global shutter |
| White balance | manual, automatic |
| manual, automatic |
| Software features | image size, brightness, exposure control |
| Software | MAGUS View |
| Output | USB 3.0, 5 Gb/s< /td> |
| System requirements | Windows 8/10/11 (32-bit and 64-bit), Mac OS Core 2 or later, minimum 2 GB RAM, USB 3.0 port, CD-ROM, 17" or larger |
| Mount type | C-mount |
| Body | CNC aluminum alloy |
| Camera power supply | DC, 5 V, from computer USB port; 12 V, 3 A adapter for the Peltier cell |
| Camera operating temperature range, °C | -10 — 50 |
| Humidity operating range, % | 30 — 80 |
